4HRO
Crystal structure of H. volcanii small archaeal modifier protein 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.928, 43.050, 61.974 |
| Unit cell angles | 90.00, 102.84, 90.00 |
Refinement procedure
| Resolution | 35.060 - 1.150 |
| R-factor | 0.14004 |
| Rwork | 0.139 |
| R-free | 0.16636 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.314 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.170 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.042 | 0.256 |
| Number of reflections | 50290 | |
| <I/σ(I)> | 35.9 | 5.8 |
| Completeness [%] | 98.6 | 89.7 |
| Redundancy | 5.9 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | 100 mM Na cacodylate (pH 6.5), 15-20% (w/v) PEG 8000, 0.2 M Ca acetate, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
