4HRH

Crystal Structure of p11-Annexin A2(N-terminal) Fusion Protein in Complex with SMARCA3 Peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Wavelength(s)	0.97920
Spacegroup name	P 43 3 2
Unit cell lengths	145.106, 145.106, 145.106
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.620 - 3.001
R-factor	0.2225
Rwork	0.220
R-free	0.26260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hrg
RMSD bond length	0.009
RMSD bond angle	1.186
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	29.620
High resolution limit [Å]	3.000
Number of reflections	10903
Completeness [%]	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.1 M MES, 1.6 M (NH4)2SO4, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K