4HRD
Crystal structure of yeast 20S proteasome in complex with the natural product carmaphycin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-02 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 134.620, 300.280, 144.570 |
| Unit cell angles | 90.00, 112.49, 90.00 |
Refinement procedure
| Resolution | 49.590 - 2.800 |
| R-factor | 0.206 |
| Rwork | 0.204 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.949 |
| Data scaling software | XSCALE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.590 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.103 | 0.481 |
| Number of reflections | 254447 | |
| <I/σ(I)> | 7.94 | 2.61 |
| Completeness [%] | 98.0 | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 297 | 30 mM of magnesium acetate, 100 mM of MES (pH 7.2) and 12% of MPD, vapor diffusion, temperature 297K |
