4HRC
Crystal structure of yeast 20S proteasome in complex with epoxyketone carmaphycin analogue 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.350, 300.880, 144.250 |
| Unit cell angles | 90.00, 112.74, 90.00 |
Refinement procedure
| Resolution | 48.000 - 2.800 |
| R-factor | 0.2119 |
| Rwork | 0.210 |
| R-free | 0.24340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.999 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.800 | |
| High resolution limit [Å] | 2.700 | 40.000 | 2.700 |
| Rmerge | 0.078 | 0.052 | 0.541 |
| Number of reflections | 284264 | 37 | 29211 |
| <I/σ(I)> | 9.33 | 23.11 | 1.76 |
| Completeness [%] | 97.8 | 35.9 | 97.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 297 | 30 mM of magnesium acetate, 100 mM of MES (pH 7.2) and 12% of MPD, vapor diffusion, temperature 297K |
