4HR5
R2-like ligand-binding oxidase without metal cofactor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-25 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.96 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 56.757, 97.043, 127.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.378 - 2.291 |
| R-factor | 0.1805 |
| Rwork | 0.178 |
| R-free | 0.23720 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.498 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.430 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.071 | 0.754 |
| Number of reflections | 16220 | |
| <I/σ(I)> | 25.87 | 4.13 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 14.6 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 17.5%(w/v) PEG 1500, 0.1M HEPES-Na, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
