4HR0
R2-like ligand-binding oxidase with aerobically reconstituted metal cofactor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-27 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.87 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 56.150, 97.738, 130.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.153 - 1.896 |
| R-factor | 0.167 |
| Rwork | 0.166 |
| R-free | 0.19560 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.244 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.896 | 1.900 |
| Rmerge | 0.054 | 0.754 |
| Number of reflections | 28825 | |
| <I/σ(I)> | 15.73 | 2 |
| Completeness [%] | 99.3 | 97.3 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 12.5%(w/v) PEG 1500, 0.1M HEPES-Na, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
