4HQK
Crystal structure of Plasmodium falciparum TRAP, P4212 form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 117.707, 117.707, 65.491 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.029 - 2.249 |
| R-factor | 0.1922 |
| Rwork | 0.187 |
| R-free | 0.23990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1shu |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.696 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.030 | 2.290 |
| High resolution limit [Å] | 2.249 | 2.249 |
| Number of reflections | 22401 | |
| <I/σ(I)> | 10.22 | 1.28 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 6.8 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 298 | 0.1 M HEPES, pH 7.5, 2% PEG400, 2 M ammonium sulfate, EVAPORATION, temperature 298K |
