4HQ0

Crystal Structure of mutant form of Caspase-7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6C1
Synchrotron site	PAL/PLS
Beamline	6C1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-08
Detector	ADSC QUANTUM 210
Wavelength(s)	1.
Spacegroup name	P 32 2 1
Unit cell lengths	89.600, 89.600, 182.840
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 3.000
R-factor	0.224
Rwork	0.222
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.380
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000
High resolution limit [Å]	3.000	3.000
Number of reflections	17689
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	sodium acetate pH 4.6, sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 293K