4HPQ

Crystal Structure of the Atg17-Atg31-Atg29 Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	200
Detector technology	CCD
Collection date	2012-06-04
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 1 21 1
Unit cell lengths	144.370, 64.200, 184.210
Unit cell angles	90.00, 110.79, 90.00

Refinement procedure

Resolution	46.560 - 3.060
R-factor	0.3048
Rwork	0.303
R-free	0.33622
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.468
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.200
High resolution limit [Å]	3.055	3.055
Rmerge	0.096	0.254
Number of reflections	47730
<I/σ(I)>	16	4.1
Completeness [%]	80.2	29
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	50 mM Tris pH8, 4-10% peg 2KMME, 10-20% ethylene glycol, 100 mM NaCL, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K
1	VAPOR DIFFUSION, HANGING DROP	8	294	50 mM Tris pH8, 4-10% peg 2KMME, 10-20% ethylene glycol, 100 mM NaCL, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K