4HPL
PCGF1 Ub fold (RAWUL)/BCOR PUFD Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-22 |
| Detector | NOIR-1 |
| Wavelength(s) | 0.919 |
| Spacegroup name | P 62 |
| Unit cell lengths | 67.681, 67.681, 75.742 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.540 - 2.000 |
| R-factor | 0.2168 |
| Rwork | 0.212 |
| R-free | 0.26060 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.156 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1161)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.540 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 13386 | |
| <I/σ(I)> | 18.5 | 4.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.1 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 295 | 50 mM MES, 8.5% polyethylene glycol (PEG) 8000, 300 mM sodium bromide, 10% ethylene glycol, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
