4HPF
Structure of the human SLO3 gating ring
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075, 1.29 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 124.540, 157.940, 249.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.510 - 3.400 |
| R-factor | 0.2477 |
| Rwork | 0.247 |
| R-free | 0.26690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mt5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.026 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 7.100 | 3.300 |
| Rmerge | 0.124 | 0.073 | |
| Number of reflections | 36517 | ||
| <I/σ(I)> | 10.5 | ||
| Completeness [%] | 99.9 | 99.3 | 100 |
| Redundancy | 8.3 | 7.7 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | PEG12000, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
