4HPA
Crystal structure of Nitrophorin 4 from Rhodnius prolixus Complexed with sulfide ion at pH 7.4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-15 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.169, 42.893, 52.255 |
| Unit cell angles | 90.00, 94.05, 90.00 |
Refinement procedure
| Resolution | 19.833 - 1.500 |
| R-factor | 0.136 |
| Rwork | 0.134 |
| R-free | 0.18140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mvf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.156 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.500 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 24703 | |
| Completeness [%] | 98.9 | 97.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | 3.2M AMMONIUM PHOSPHATE, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
