4HOU

Crystal Structure of N-terminal Human IFIT1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	105
Detector technology	CCD
Collection date	2010-01-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	84.470, 176.952, 55.143
Unit cell angles	90.00, 130.13, 90.00

Refinement procedure

Resolution	30.521 - 1.950
R-factor	0.2036
Rwork	0.202
R-free	0.23240
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.138
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.020
High resolution limit [Å]	1.950	1.950
Number of reflections	45919
Completeness [%]	98.0	95.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295.15	pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K