4HOS

Crystal Structure of Full-Length Human IFIT5 with 5`-triphosphate Oligouridine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	105
Detector technology	CCD
Collection date	2012-07-15
Detector	RIGAKU SATURN 944+
Spacegroup name	P 1 21 1
Unit cell lengths	54.475, 84.939, 60.928
Unit cell angles	90.00, 106.75, 90.00

Refinement procedure

Resolution	31.790 - 2.000
R-factor	0.177
Rwork	0.175
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.951
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	36176
Completeness [%]	99.9	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	295.15	pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K