4HOS
Crystal Structure of Full-Length Human IFIT5 with 5`-triphosphate Oligouridine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2012-07-15 |
| Detector | RIGAKU SATURN 944+ |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.475, 84.939, 60.928 |
| Unit cell angles | 90.00, 106.75, 90.00 |
Refinement procedure
| Resolution | 31.790 - 2.000 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.951 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 36176 | |
| Completeness [%] | 99.9 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 295.15 | pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K |
