4HO1
The Crystal structure of Haemophilus influenzae O-acetylserine sulfhydrylase at 1.85A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-04-06 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 41 |
| Unit cell lengths | 112.786, 112.786, 46.297 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.670 - 1.856 |
| R-factor | 0.14076 |
| Rwork | 0.140 |
| R-free | 0.16311 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y7l |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 79.750 |
| High resolution limit [Å] | 1.856 |
| Rmerge | 0.044 |
| Number of reflections | 24451 |
| <I/σ(I)> | 34.81 |
| Completeness [%] | 97.7 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | 0.2M Magnesium chloride,0.1M tris HCL pH 8.5, 30% w/v PEG 4000 , VAPOR DIFFUSION, SITTING DROP, temperature 292K |
