4HNX

The NatA Acetyltransferase Complex Bound To ppGpp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97937, 1.0
Spacegroup name	P 31 2 1
Unit cell lengths	137.105, 137.105, 179.603
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.144 - 2.339
R-factor	0.2144
Rwork	0.213
R-free	0.23970
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.015
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.339	2.340
Number of reflections	82175
<I/σ(I)>		2
Completeness [%]	99.3	99.4
Redundancy	6.2	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.3	291	1-6% Jeffamine ED-2001, pH 7.0; 0.1 M K-HEPES pH 6.3, 0.1-2% PEG200, VAPOR DIFFUSION, SITTING DROP, temperature 291K