4HNW

The NatA Acetyltransferase Complex Bound To Inositol Hexakisphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97937
Spacegroup name	P 31 2 1
Unit cell lengths	135.662, 135.662, 175.801
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.487 - 2.801
R-factor	0.23
Rwork	0.229
R-free	0.25090
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.640
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.119
Number of reflections	46561
<I/σ(I)>	7	2.7
Completeness [%]	99.9	99.8
Redundancy	15.7	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.9	291	0.1 M sodium acetate pH 4.9, 0.175 M ammonium acetate, 3-5% PEG4000, 0.5-4% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	4.9	291	0.1 M sodium acetate pH 4.9, 0.175 M ammonium acetate, 3-5% PEG4000, 0.5-4% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 291K