4HNP
Crystal structure of yeast 20S proteasome in complex with vinylketone carmaphycin analogue VNK1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.850, 301.360, 144.460 |
| Unit cell angles | 90.00, 112.82, 90.00 |
Refinement procedure
| Resolution | 48.420 - 2.800 |
| R-factor | 0.2048 |
| Rwork | 0.203 |
| R-free | 0.23890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.950 |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.420 | 2.900 | |
| High resolution limit [Å] | 2.800 | 40.000 | 2.800 |
| Rmerge | 0.096 | 0.069 | 0.485 |
| Number of reflections | 257429 | 38 | 25628 |
| <I/σ(I)> | 8.76 | 17.6 | 2.56 |
| Completeness [%] | 98.2 | 36.9 | 97.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 297 | 30 mM of magnesium acetate, 100 mM of MES (pH 7.2) and 12% of MPD, vapor diffusion, temperature 297K |
