4HNN
Dihydrodipicolinate Synthase from the common grapevine with pyruvate and lysine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Wavelength(s) | 0.9536 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 220.610, 137.930, 133.940 |
| Unit cell angles | 90.00, 108.25, 90.00 |
Refinement procedure
| Resolution | 127.200 - 2.400 |
| R-factor | 0.18175 |
| Rwork | 0.181 |
| R-free | 0.21621 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.198 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 127.200 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 146233 |
| Completeness [%] | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M MES pH 6.5, 6% (v/v) PEG 20,000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
