4HNM

Crystal structure of human catenin-beta-like 1 56 kDa fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-E
Synchrotron site	APS
Beamline	21-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97872
Spacegroup name	P 41 21 2
Unit cell lengths	94.970, 94.970, 172.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.245 - 2.900
R-factor	0.2122
Rwork	0.207
R-free	0.25760
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.687
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.245	34.526	2.950
High resolution limit [Å]	2.800	8.850	2.800
Rmerge		0.039	0.775
Number of reflections	20205
<I/σ(I)>	23.4	14.9	1
Completeness [%]	99.9	98.2	100
Redundancy	19.8	16.3	19.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	PEG 2000, Tris 8.0, KBr, vapor diffusion, hanging drop, temperature 298K