4HNL
Crystal structure of ENOLASE EGBG_01401 (TARGET EFI-502226) from Enterococcus gallinarum EG2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-21 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 115.943, 115.943, 119.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.640 - 1.480 |
| R-factor | 0.12445 |
| Rwork | 0.123 |
| R-free | 0.16215 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gy1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.248 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0025) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.510 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.690 | |
| Number of reflections | 67893 | |
| <I/σ(I)> | 8.5 | 4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 29 | 28.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.2M MAGNESIUM CHLORIDE, PH 8.5, 0.1M TRIS-HCL, 16% PEG MME4000, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |
