4HNB

Crystal Structure of Rhodobacter Sphaeroides LOV protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-19
Detector	ADSC QUANTUM 210
Wavelength(s)	0.987
Spacegroup name	P 65
Unit cell lengths	109.346, 109.346, 85.479
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.956 - 2.340
R-factor	0.2075
Rwork	0.204
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.956	2.380
High resolution limit [Å]	2.340	2.340
Number of reflections	21851
Completeness [%]	88.7	93.1
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	290	0.1 M HEPES, 4.3 M NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K