4HN7
Crystal structure of E. coli PmrD
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97959 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 83.580, 83.580, 75.951 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 16.810 - 2.352 |
| R-factor | 0.21435 |
| Rwork | 0.211 |
| R-free | 0.28145 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jso |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.160 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.520 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.370 | 2.000 |
| Number of reflections | 5617 | ||
| Completeness [%] | 79.6 | 97.2 | 28.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 50 mM sodium acetate, 2.0 M ammonium sulfate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
