4HMX

Crystal structure of PhzG from Burkholderia lata 383 in complex with tetrahydrophenazine-1-carboxylic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-02
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.99988
Spacegroup name	P 31
Unit cell lengths	93.650, 93.650, 51.410
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.825 - 1.590
R-factor	0.1149
Rwork	0.113
R-free	0.15760
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.019
RMSD bond angle	1.702
Data scaling software	XSCALE
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.000
High resolution limit [Å]	1.590
Number of reflections	67792

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	16-20% (w/v) PEG 3350, 0.1M trisodium citrate, 15uM PhzF, 30uM PhzA/B, 0.2M TRIS-HCl pH 7.5 , vapor diffusion, hanging drop, temperature 298K