4HMW

Crystal structure of PhzG from Burkholderia lata 383

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.99988
Spacegroup name	P 31
Unit cell lengths	94.416, 94.416, 51.751
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.729 - 1.530
R-factor	0.1352
Rwork	0.133
R-free	0.17880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.360
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.000
High resolution limit [Å]	1.530
Number of reflections	77682

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	16-20% (w/v) PEG 3350, 0.1 M trisodium citrate, pH 5.2-5.6, vapor diffusion, hanging drop, temperature 298K