4HMV

Crystal structure of PhzG from Pseudomonas fluorescens 2-79 in complex with tetrahydrophenazine-1-carboxylic acid after 5 days of soaking

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-16
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.98004
Spacegroup name	P 21 21 21
Unit cell lengths	57.668, 63.575, 133.224
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.599 - 1.450
R-factor	0.1198
Rwork	0.118
R-free	0.15030
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.010
RMSD bond angle	1.322
Data scaling software	XSCALE
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	44.000
High resolution limit [Å]	1.450
Number of reflections	87433

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	21-24% (w/v) PEG 3350, 0.2M (NH4)2SO4, 0.1M BisTris, 15uM PhzF, 30uM PhzA/B, 0.2M TRIS-HCl pH 7.5 , vapor diffusion, hanging drop, temperature 298K