4HMS
Crystal structure of PhzG from Pseudomonas fluorescens 2-79 in complex with a second FMN in the substrate binding site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-19 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.740, 63.690, 133.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.278 - 1.330 |
| R-factor | 0.1224 |
| Rwork | 0.121 |
| R-free | 0.15350 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.575 |
| Data scaling software | SCALA |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.285 |
| High resolution limit [Å] | 1.271 |
| Number of reflections | 113420 |
| Completeness [%] | 99.8 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 21-24% (w/v) PEG 3350, 0.2M (NH4)2SO4, 0.1M BisTris, 10mM FMN, pH 6.2-6.6, vapor diffusion, hanging drop, temperature 298K |
