4HMK
Crystal structure of LeuT-E290S with bound Br
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.94 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.140, 92.320, 87.210 |
| Unit cell angles | 90.00, 94.25, 90.00 |
Refinement procedure
| Resolution | 63.304 - 3.000 |
| R-factor | 0.208 |
| Rwork | 0.205 |
| R-free | 0.26090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.183 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 26770 | |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 24% PEG 550MME, 0.6M NaBr, 0.1M Hepes, pH 7.0, 100nM Leu, 2.4% w/v OG, VAPOR DIFFUSION, sitting drop, temperature 292K |
