4HM9

Crystal structure of full-length human catenin-beta-like 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-E
Synchrotron site	APS
Beamline	21-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	48.990, 116.360, 137.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.618 - 3.100
R-factor	0.1772
Rwork	0.174
R-free	0.22530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.597
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.145	46.145	2.530
High resolution limit [Å]	2.399	7.590	2.399
Rmerge		0.035	0.035
Number of reflections	31661
<I/σ(I)>	6.1	9.9	0.2
Completeness [%]	100.0	99.6	99.9
Redundancy	6.7	6.3	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	PEG 2000, Tris 8.0, KBr, vapor diffusion, hanging drop, temperature 298K