4HLX
The crystal structure of the DNA binding domain of vIRF-1 from the oncogenic KSHV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2011-11-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.96722 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 65.770, 128.843, 228.462 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.292 - 2.379 |
| R-factor | 0.2525 |
| Rwork | 0.251 |
| R-free | 0.28620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.567 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.292 | |
| High resolution limit [Å] | 2.379 | 2.380 |
| Number of reflections | 18285 | |
| Completeness [%] | 92.5 | 92.58 |
| Redundancy | 6.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1 M HEPES pH 7, 27% Jeffamine-ED 2001, 10 mM ATP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
