4HLQ

Crystal structure of human rab1b bound to GDP and BEF3 in complex with the GAP domain of TBC1D20 from homo sapiens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-03-25
Detector	PSI PILATUS 6M
Wavelength(s)	0.9999
Spacegroup name	P 21 21 21
Unit cell lengths	88.170, 118.640, 290.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.220 - 3.300
R-factor	0.20937
Rwork	0.206
R-free	0.27432
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hl4 3nkv
RMSD bond length	0.007
RMSD bond angle	1.057
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (MR)
Refinement software	REFMAC (5.5.0070)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.400
High resolution limit [Å]	3.300	3.300
Rmerge	0.056
Number of reflections	46667
<I/σ(I)>	3.31
Completeness [%]	100.0	100
Redundancy	6.63	6.47

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	293	1.6M ammonium sulphate, 0.1M Hepes pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K