4HLJ

Axon Guidance Receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-05
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97626
Spacegroup name	P 21 21 21
Unit cell lengths	28.700, 77.710, 91.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.950 - 1.800
R-factor	0.1866
Rwork	0.184
R-free	0.21140
Structure solution method	SAD-MR
RMSD bond length	0.003
RMSD bond angle	0.770
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.950	1.840	1.700
High resolution limit [Å]	1.670	1.800	1.670
Rmerge	0.045
Number of reflections	21364
Completeness [%]	86.0	100	86.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	35% PEG 400, 0.1M Sodium acetate trihydrate, 5% glycerol, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K