4HL8

Re-refinement of the vault ribonucleoprotein particle

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2007-12-24
Detector	Bruker DIP-6040
Wavelength(s)	0.9
Spacegroup name	C 1 2 1
Unit cell lengths	702.246, 383.796, 598.480
Unit cell angles	90.00, 124.69, 90.00

Refinement procedure

Resolution	200.000 - 3.500
R-factor	0.352
Rwork	0.352
R-free	0.35400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	1.007
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	204.000	3.690
High resolution limit [Å]	3.500	3.500
Rmerge	0.233
Number of reflections	1531361
<I/σ(I)>	5.7	1.2
Completeness [%]	92.4	81.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	288	2.4% PEG 4000, 0.8M sodium chloride, 0.05M lithium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K