4HKW
Crystal Structures of Mutant Endo-beta-1,4-xylanase II Complexed with Substrate and Products
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-06-01 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5417 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.879, 38.428, 56.315 |
Unit cell angles | 90.00, 105.93, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.650 |
R-factor | 0.1587 |
Rwork | 0.155 |
R-free | 0.21910 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.025 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 1.650 |
Number of reflections | 16432 |
Completeness [%] | 84.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M TRIS, 0.1M CaCl2, pH=8.5, 15% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |