4HKE

Crystal Structure of MoxT of Bacillus anthracis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-06-01
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	43.163, 70.195, 79.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.610 - 1.870
R-factor	0.18518
Rwork	0.182
R-free	0.22456
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ne8
RMSD bond length	0.014
RMSD bond angle	1.631
Data reduction software	AUTOMAR
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	52.630
High resolution limit [Å]	1.870
Number of reflections	19003

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	2M Ammonium sulfate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K