4HK7
Crystal structure of Cordyceps militaris IDCase in complex with uracil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0600 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.333, 144.921, 77.310 |
| Unit cell angles | 90.00, 97.14, 90.00 |
Refinement procedure
| Resolution | 76.730 - 2.189 |
| R-factor | 0.1557 |
| Rwork | 0.153 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hk5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.103 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 76.733 | 50.000 | 2.280 |
| High resolution limit [Å] | 2.189 | 4.740 | 2.189 |
| Rmerge | 0.085 | 0.041 | 0.481 |
| Number of reflections | 86105 | ||
| <I/σ(I)> | 10 | ||
| Completeness [%] | 99.5 | 99.6 | 96.5 |
| Redundancy | 3.4 | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 25% PEG3350, 0.2 M magnesium chloride, 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
