4HK3
I2 Fab (unbound) from CH65-CH67 Lineage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.99997 |
| Spacegroup name | P 64 |
| Unit cell lengths | 102.455, 102.455, 81.492 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.964 - 3.000 |
| R-factor | 0.2239 |
| Rwork | 0.221 |
| R-free | 0.28960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.293 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.498 |
| Rmerge | 0.060 |
| Number of reflections | 16968 |
| <I/σ(I)> | 8.4 |
| Completeness [%] | 100.0 |
| Redundancy | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 1.5 M sodium citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
