4HJI
Structure of the CooA pilin subunit from enterotoxigenic Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-08 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.07 |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.990, 46.870, 51.270 |
| Unit cell angles | 83.72, 89.95, 74.58 |
Refinement procedure
| Resolution | 19.670 - 1.600 |
| R-factor | 0.22356 |
| Rwork | 0.222 |
| R-free | 0.25057 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f83 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.374 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.025 | |
| Number of reflections | 33503 | |
| <I/σ(I)> | 34.1 | |
| Completeness [%] | 92.2 | 92.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1 M SODIUM CITRATE, 100 MM IMIDAZOLE , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
