4HJC
Crystal structure of glycoprotein C from Rift Valley Fever Virus (non-glycosylated)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 114.347, 114.347, 170.675 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.988 - 4.150 |
| R-factor | 0.2617 |
| Rwork | 0.259 |
| R-free | 0.31120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.798 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 4.220 |
| High resolution limit [Å] | 4.150 | 11.190 | 4.150 |
| Rmerge | 0.075 | 0.037 | |
| Number of reflections | 5438 | ||
| <I/σ(I)> | 5 | ||
| Completeness [%] | 99.8 | 98.2 | 100 |
| Redundancy | 12.6 | 10.3 | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 289 | 12% PEG 5000 MME, 0.1 M MES, O.1 M Ammonium sulfate, 5% glycerol, 1.8 mM UDM, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
