4HJA

Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssDNA (ACGGTTACGGT)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-12-09
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	45.850, 56.776, 66.258
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.408 - 2.100
R-factor	0.1983
Rwork	0.193
R-free	0.24790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hik
RMSD bond length	0.008
RMSD bond angle	1.225
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX (1.7_650)
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.081	0.041	0.463
Number of reflections	12182
<I/σ(I)>	8.5
Completeness [%]	99.3	99.5	93.4
Redundancy	3.4	3.3	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	25% PEG 8000, 0.2M ammonium formate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 290K