4HJ6

Crystal Structure of Rhodobacter Sphaeroides LOV protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Collection date	2011-10-19
Spacegroup name	P 65
Unit cell lengths	108.134, 108.134, 84.632
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.216 - 2.200
R-factor	0.2079
Rwork	0.206
R-free	0.22560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.161
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.079	0.054	0.585
Number of reflections	28665
<I/σ(I)>	12.7
Completeness [%]	100.0	99.3	100
Redundancy	11.3	10.6	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	290	0.1 M HEPES, 4.3 M NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K