4HJ3

Crystal Structure of Rhodobacter Sphaeroides LOV protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Collection date	2012-03-26
Wavelength(s)	0.978
Spacegroup name	P 65
Unit cell lengths	105.784, 105.784, 83.983
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.806 - 2.643
R-factor	0.1958
Rwork	0.191
R-free	0.24030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.253
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.640	7.160	2.640
Rmerge	0.075	0.039	0.511
Number of reflections	15765
<I/σ(I)>	13.2
Completeness [%]	100.0	100	100
Redundancy	10.7	10.6	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	290	0.1 M HEPES, 4.3 M NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K