4HIZ
Phage phi92 endosialidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.858, 137.584, 140.778 |
| Unit cell angles | 90.00, 94.64, 90.00 |
Refinement procedure
| Resolution | 19.968 - 1.601 |
| R-factor | 0.1074 |
| Rwork | 0.107 |
| R-free | 0.14680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.523 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.520 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.042 | 0.269 |
| Number of reflections | 305050 | |
| <I/σ(I)> | 17.1 | 4.3 |
| Completeness [%] | 99.1 | 97.7 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 291 | 6% PEG 3350 70 mM CHES pH 9.5 70 mM Strontium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
