4HIU
Crystal structure of R34/53A mutant of borna disease virus matrix protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 139.732, 139.732, 139.732 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.300 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f1j |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.474 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.090 | 0.703 |
| Number of reflections | 3751 | |
| <I/σ(I)> | 25.5 | 3.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.09 M NPS buffer, 30% GLYCEROL, PEG4000, 0.1 M HEPES (MOPS), pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
