4HIN
2.4A Resolution Structure of Bovine Cytochrome b5 (S71L)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.530, 92.709, 48.990 |
| Unit cell angles | 90.00, 113.11, 90.00 |
Refinement procedure
| Resolution | 46.350 - 2.400 |
| R-factor | 0.2104 |
| Rwork | 0.209 |
| R-free | 0.24510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ehb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | SCALA (CCP4_3.3.16) |
| Phasing software | MOLREP |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 92.710 | 92.710 | 2.530 |
| High resolution limit [Å] | 2.400 | 7.590 | 2.400 |
| Rmerge | 0.128 | 0.040 | 0.660 |
| Total number of observations | 1592 | 7792 | |
| Number of reflections | 15234 | ||
| <I/σ(I)> | 7.8759 | 2.3 | |
| Completeness [%] | 99.2 | 97.12 | 99.5 |
| Redundancy | 3.41 | 3.2 | 3.51 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 20% (w/v) PEG 8000, 100 mM Tris, 200 mM MgCl2, 10 mM CuCl2, pH 8.5, vapor diffusion, temperature 293K |
