4HIA
Crystal Structure of Rhodobacter Sphaeroides LOV protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.356, 73.183, 95.288 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.644 - 1.952 |
| R-factor | 0.175 |
| Rwork | 0.173 |
| R-free | 0.20120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.096 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 5.290 | 1.950 |
| Rmerge | 0.092 | 0.048 | 0.395 |
| Number of reflections | 28328 | ||
| <I/σ(I)> | 9.2 | ||
| Completeness [%] | 99.6 | 99.9 | 90.9 |
| Redundancy | 13.8 | 13.1 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 290 | LiSO4, 0.1 M HEPES, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
