4HI1
Crystal structure of acylphosphatase C20R mutant from Vibrio cholerae0395
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-07-13 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.710, 81.300, 40.530 |
| Unit cell angles | 90.00, 90.52, 90.00 |
Refinement procedure
| Resolution | 39.708 - 1.964 |
| R-factor | 0.1907 |
| Rwork | 0.187 |
| R-free | 0.24870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bjd |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.328 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.708 | 2.060 |
| High resolution limit [Å] | 1.964 | 1.960 |
| Rmerge | 0.062 | |
| Number of reflections | 17237 | |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 93.5 | 93.5 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 1.6M ammonium sulfate, 0.1M bicine pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
