4HI0

Crystal Structure of Helicobacter pylori Urease Accessory Protein UreF/H/G complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	76.124, 96.429, 237.452
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.875 - 2.350
R-factor	0.1929
Rwork	0.192
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.187
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.490
High resolution limit [Å]	2.350	2.350
Number of reflections	71616
Completeness [%]	99.5	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	289	1.5M ammonium sulfate, 100mM sodium acetate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K