4HHX
Structure of cytoplasmic domain of TCPE from Vibrio cholerae
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.96109, 0.97905 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 34.790, 73.610, 95.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.630 - 1.880 |
| R-factor | 0.21972 |
| Rwork | 0.218 |
| R-free | 0.24739 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.014 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SQUASH |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.530 | 1.930 |
| High resolution limit [Å] | 1.880 | 2.420 | 1.880 |
| Number of reflections | 10378 | ||
| Completeness [%] | 99.5 | 99.8 | 96.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 100 mM Tris-HCl, 1.55 M ammonium sulfate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
