4HHA
Anti-Human Cytomegalovirus (HCMV) Fab KE5 with epitope peptide AD-2S1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2012-09-28 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.250, 65.510, 67.110 |
| Unit cell angles | 90.00, 96.58, 90.00 |
Refinement procedure
| Resolution | 43.000 - 1.600 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hh9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.600 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.048 | 0.492 |
| Number of reflections | 48944 | |
| <I/σ(I)> | 19.84 | 2.89 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 3.72 | 3.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 298 | 0.05M Na Acetate, pH 4.2, 18% Jeffamine 1500, 0.2M Ammonium chloride , VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
